Ana Pio wrote and directed her debut short film, in 2014, I Welcome You to This House, financed by the Portuguese Institute of Cinema and Audiovisual.

In 2015, she graduated with the short period drama Eden from her Directing Masters at London Met Film School. Eden screened in several 2nd tier international film festivals, won the Women in Film Achievement Award at the New Renaissance Film Festival, was broadcasted on RTP 2 TV and distributed by Shorts International.

From the Sara Putt Associates trainee scheme in 2017, Ana Pio became a freelance Script Supervisor, working on iFeatures Perfect 10 and assisting the Script Supervisors on BBC/Amazon TV series Good Omens alongside Douglas MacKinnon and Neil Gaiman as well as Liaison directed by Stephen Hopkins, starring Eva Green and Vincent Cassel.

Ana is currently a BFI NETWORK x BAFTA Crew member, distributing her comedy short film PMS, as well as in post-production of short films Do As I Say and 7 to 10 with producers Andrea Land, Gerry Maguire, Gemma Albone and Kate Shelley whilst developing her debut feature film Mother Nature mentored by Rose Glass, the director of Saint Maud, through the Directors UK Inspire programme.