Ana Pio wrote and directed her debut short film, in 2014, I Welcome You to This House, financed by the Portuguese Institute of Cinema and Audiovisual.

In 2015, she graduated with the short period drama Eden from her Directing Masters at London Met Film School. Eden screened in over 25 international film festivals, won the Women in Film Achievement Award at the New Renaissance Film Festival, broadcasted on RTP 2 TV - Rádio e Televisão de Portugal - and is currently being distributed by Shorts International.

From the Sara Putt Associates trainee scheme in 2017 Trainee Scheme, Ana Pio became a freelance Script Supervisor, working on iFeatures Perfect 10 and assisting the Script Supervisors on BBC/Amazon TV series Good Omens alongside Douglas MacKinnon and Neil Gaiman as well as Liaison directed by Stephen Hopkins, starring Eva Green and Vincent Cassel.

Ana is currently a BFI NETWORK x BAFTA Crew member, distributing her comedy short film PMS, as well as in post-production of short film Do As I Say with producers Andrea Land and Gerry Maguire and pre-production of 7 to 10 whilst developing her debut feature film Mother Nature mentored by Rose Glass, the director of Saint Maud, through the Directors UK Inspire programme.